3XFLAG peptide The FLAG peptide, also known by its amino acid sequence DYKDDDDK, is a widely recognized and utilized eight-amino acid epitope tag in molecular biology research更新日期 2025-05-22; 产品编号 HY-P0319; 品牌; 产品信息 3X FLAG PEPTIDE3X FLAG peptide; CAS号 402750-12-3; MDL号; 规格与纯度; 包装 1mg; 价格 600 .... This short, hydrophilic, and highly charged peptide sequence plays a crucial role in the detection and purification of recombinant fusion proteins. Its versatility makes it an indispensable tool for scientists aiming to express, purify, and analyze proteins of interest.FLAG peptide是一种八个氨基酸组成的多肽(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)，具有肠激酶切割位点，可用于抗体介导的重组蛋白的识别和纯化。
The FLAG peptide is an artificial tag, meaning it is not derived from a naturally occurring protein.FLAG peptide | CAS 98849-88-8 | Selleck It was specifically designed to facilitate the identification and isolation of proteins to which it is attachedDYKDDDDKpeptideis an eight-amino-acid (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) epitope tag used for protein purification and detection.. The DYKDDDDK sequence is key to its function, allowing for highly specific interactions with anti-FLAG antibodies. This specificity is vital for applications such as affinity purification, Western blotting, and immunoprecipitation.
The primary utility of the FLAG peptide lies in its ability to enable the purification of recombinant proteins. When a FLAG tag is incorporated into a protein's sequence through recombinant DNA technology, it acts as a handle for purificationFLAG peptide | FLAG标记肽. Antibodies that specifically recognize the FLAG epitope can be used to capture the tagged protein from a complex mixture. This is often achieved using anti-FLAG agarose resins or magnetic beads.
A significant advantage of the FLAG tag system is its ability to allow for elution of the purified proteins under non-denaturing conditions. This is particularly important for maintaining the biological activity of sensitive proteins. The FLAG peptide itself can be used as a competitor to gently elute the bound fusion proteins from the antibody affinity matrix, preserving their native conformation and function碧云天3X Flag Peptide (3X Flag多肽)(P9801-25mg).
Furthermore, the FLAG tag is valuable for detectionFLAG tag Peptideis an 8-peptide (Asp-Tyr-lys-Asp-Asp-Asp-ASP-ASP-Lys) containing an intestinal kinase restriction site. FLAG Tag peptide fusion system is .... Anti-FLAG antibodies can be used in techniques like Western blotting to confirm the presence and size of the tagged protein, even when other detection methods may not be effective. The tag's small size and hydrophilic nature generally minimize interference with the protein's natural folding and function.FLAG peptide是一种用于重组蛋白纯化的多功能融合标签。FLAG peptide 维持其融合蛋白的自然折叠。FLAG peptide 可被肠激酶去除，并在非变性条件下洗脱。
The standard FLAG peptide consists of the eight-amino acid sequence Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK).3X FLAG PEPTIDE价格(试剂级) This sequence contains an enterokinase cleavage site, which allows for the removal of the FLAG tag after purification if desired, further ensuring that the final protein product is free from the tag.
Variations of the FLAG tag, such as the 3x FLAG peptide, also exist. These versions typically involve repeating the FLAG sequence multiple times (e多肽标签FLAG peptide DYKDDDDK|98849-88-8.g., DYKDDDDK-DYKDDDDK-DYKDDDDK)更新日期 2025-05-22; 产品编号 HY-P0319; 品牌; 产品信息 3X FLAG PEPTIDE3X FLAG peptide; CAS号 402750-12-3; MDL号; 规格与纯度; 包装 1mg; 价格 600 .... The increased number of epitope sites in a 3x FLAG tag can potentially enhance binding affinity to anti-FLAG antibodies, leading to more efficient purification or detection, especially for low-expression proteins. While the core functionality remains the same, these multi-tag versions can offer improved performance in certain experimental setups.
When utilizing the FLAG peptide system, several factors are important to consider. The choice of anti-FLAG antibody (e.g.The FLAG™ peptide, a versatile fusion tag for ..., M1, M2, M5) can influence the efficiency of binding and elution, as different antibodies may have varying affinities or recognize different epitopes within the FLAG sequence.The FLAG peptide, FLAG tag or FLAG epitopeallows highly specific pull-downs with a low background. The FLAG peptide is used in many different assays such ... The conditions for elution, especially whether they are denaturing or non-denaturing, must be chosen based on the specific protein being studied and its sensitivity.
The FLAG peptide is a powerful tool in the molecular biologist's arsenal, enabling streamlined protein expression, purification, and detection.多肽标签FLAG peptide DYKDDDDK|98849-88-8 Its well-defined sequence, high specificity, and the availability of various antibody and tag formats contribute to its widespread adoption and continued relevance in scientific research.
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