aromazone-peptide-de-poid The Ascentis Express Peptide ES-C18 is a specialized high-performance liquid chromatography (HPLC) column designed for the efficient separation of higher molecular weight compounds, particularly peptides and small proteins. This column utilizes Fused-Core particle technology, featuring a unique 160 Å pore size and a sterically protected C18 bonded phase. This design offers a stable reversed-phase packing with high column efficiency and excellent resistance to acid-catalyzed hydrolysis, making it a robust choice for demanding analytical applications.
The Ascentis Express Peptide ES-C18 column stands out due to its specific engineering for peptide analysis. The Fused-Core particle technology, with its 2.7 µm particle size and 160 Å pores, provides superior efficiency and faster analysis times compared to traditional fully porous particles. The sterically protected C18 phase ensures longevity and reliability, even under challenging mobile phase conditions. These attributes collectively contribute to high-resolution separations, making it ideal for applications involving peptides and small proteins where precise characterization is crucialMerck: Ascentis® Express Products: Columns used for ....
At the core of the Ascentis Express Peptide ES-C18 column's performance is its innovative particle design.ASCENTIS EXPRESS C18 The Fused-Core particles consist of a solid, non-porous core surrounded by a thin porous layerHC-C18(2) and TC-C18(2) HPLC Columns | Low Cost - Agilent. This structure allows for higher column efficiency by minimizing band broadening, while the larger 160 Å pore size is specifically tailored to accommodate larger molecules like peptides. The 2.7 µm particle size further enhances efficiency and throughput, enabling faster analyses without compromising resolution.MilliporeSigma Supelco Ascentis Express Peptide ES-C18 ... The C18 bonded phase, being sterically protected, offers enhanced chemical stability, particularly against acidic mobile phases commonly encountered in peptide separations.
The primary application of the Ascentis Express Peptide ES-C18 column lies in the separation and analysis of peptides and small proteins.Ascentis® Express Peptide ES-C18, 2.7 μm Guard ... Its optimized pore size and bonded phase make it exceptionally well-suited for tasks such as peptide mapping, protein digestion analysis, and the characterization of biopharmaceuticals.Ascentis® Express 2.0 μm C18 Column Care & Use Sheet The column's ability to deliver high resolution and reproducible results is critical for researchers and analysts working in fields like proteomics, pharmaceutical development, and quality control, where accurate identification and quantification of these complex molecules are paramountHC-C18(2) and TC-C18(2) HPLC Columns | Low Cost - Agilent.
While C18 columns are a staple in reversed-phase chromatography, the Ascentis Express Peptide ES-C18 distinguishes itself through its specialized design for larger analytes. Compared to standard C18 columns with smaller pore sizes (e.g.SUPELCO,ASCENTIS EXPRESS PEPTIDE ES C18 15CM ..., 90 Å), the 160 Å pore size of the Peptide ES-C18 is better suited for peptides and small proteins, preventing their exclusion from the pores and ensuring optimal retention and separationAscentis Express HPLC Resource Guide. Furthermore, its Fused-Core technology provides advantages in terms of efficiency and speed over traditional fully porous particles. Other specialized columns, such as those designed for amines or small molecules, would offer different selectivity and performance characteristics not optimized for peptide analysis.
The Ascentis Express Peptide ES-C18 columns are available from various suppliers, including MilliporeSigma (Supelco) and other laboratory equipment distributors. They come in different dimensions, such as 15 cm x 4Available in 2.7 µm and 5 µm sizes.Columns used for seperation of peptides, amines and small proteins. Peptide ES-C18 HPLC Columns Separate high molecular ....6 mm or 2.1 mm x 150 mm, and often in particle sizes of 2.Ascentis ® Express Peptide ES-C18 (2.7 μm) HPLC Column7 µm. Specific product numbers, like 53307-U or 53301-U, are used for identificationAscentis Express Peptide ES-C1 | 53314-U | SUPELCO | SLS. Guard cartridges are also available to protect the analytical column and extend its lifespan. Pricing can vary depending on the supplier, dimensions, and quantity, with typical costs ranging from several hundred to over a thousand dollars per column.
In conclusion, the Ascentis Express Peptide ES-C18 column is a high-performance chromatography tool engineered for the specific challenges of peptide and small protein separationsAscentis® Express 2.0 μm C18 Column Care & Use Sheet. Its advanced Fused-Core particle technology, larger pore size, and robust C18 phase provide excellent efficiency, speed, and stability, making it a valuable asset for researchers and analysts in demanding scientific applications.
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